Journal: Biology of Sex Differences

Article Title: Sex differences in disease severity and immune responses in murine and human inflammatory arthritis

doi: 10.1186/s13293-026-00840-w

Figure Lengend Snippet: Male CIA mice exhibit higher disease severity compared to females. Male and female CIA and saline control mice were monitored for disease severity and assigned clinical scores from day 21 after the first CII challenge until the end of experiment (day 29). (A) Line graphs representing the mean clinical scores of mice starting from day 1 to day 29. On day 29 after the first CII challenge, mice were euthanized by cardiac puncture under anesthesia for blood and serum collection and storage at −80 °C. Serum concentrations of (B) anti-mouse collagen type II antibodies (left panel) and anti-bovine collagen type II antibodies (right panel) were analyzed by ELISA. N = 5 mice per group. One of two independent experiments. Simple linear regression analysis was performed to determine the statistical difference between the lines. One-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison post hoc test was used to determine the statistical significance between the groups. (*/ # p ≤ 0.05, ** p ≤ 0.005 and ***/ ### p ≤ 0.0005). In Fig. 1A, # significance of comparisons between CIA and saline control mice; *significance of comparisons between sexes of CIA mice

Article Snippet: Serum levels of mouse anti-collagen antibodies (autoantibodies) and bovine anti-collagen antibodies (antibodies to the immunizing antigen) were determined by ELISA using a Mouse Anti-mouse Type II Collagen IgG Antibody Assay Kit and Mouse Anti-Bovine Type II Collagen IgG Antibody Assay Kit, respectively, according to the manufacture’s protocol (Chondrex Inc. WA, USA).

Techniques: Saline, Control, Enzyme-linked Immunosorbent Assay, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Targeting of Phospholipase D1 Ameliorates Collagen-Induced Arthritis via Modulation of Treg and Th17 Cell Imbalance and Suppression of Osteoclastogenesis

doi: 10.3390/ijms21093230

Figure Lengend Snippet: PLD1 deficiency suppresses the collagen type II-specific humoral response and the production of proinflammatory cytokines in collagen-induced arthritis (CIA) mice. ( A ) The amount of anti-collagen total IgG, IgG1, and IgG2a antibodies was measured by ELISA in the serum from the indicated mice at day 42. ( B ) Measurement of proinflammatory cytokines in the serum of the indicated mice at day 42 as analyzed by ELISA. n = 15 per group. * p < 0.05, N.S., non-significant. Results are shown as mean ± standard error of the mean (SEM).

Article Snippet: The amount of murine CII-specific autoantibodies in the serum was measured using the Mouse Anti-Type II Collagen IgG Subtype Antibody Assay Kit (Chondrex), according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Targeting of Phospholipase D1 Ameliorates Collagen-Induced Arthritis via Modulation of Treg and Th17 Cell Imbalance and Suppression of Osteoclastogenesis

doi: 10.3390/ijms21093230

Figure Lengend Snippet: The PLD1 inhibitor reduces the production of the anti-CII IgG2a autoantibody and proinflammatory cytokines in collagen-induced arthritis (CIA) mice. ( A ) The amount of anti-collagen IgG1 and IgG2a antibodies was measured by ELISA in the serum of the indicated mice at day 45. ( B ) The amount of proinflammatory cytokines was measured by ELISA in the serum of the indicated mice at day 45. n = 15 per group. * p < 0.05. Results are shown as mean ± standard error of the mean (SEM).

Article Snippet: The amount of murine CII-specific autoantibodies in the serum was measured using the Mouse Anti-Type II Collagen IgG Subtype Antibody Assay Kit (Chondrex), according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay